PEG-8000慢病毒浓缩液
产品名称： PEG-8000慢病毒浓缩液
英文名称： PEG-8000 Lentivirus Concentration Solution
产品编号： JY03013
产品价格：
产品产地：
品牌商标： 江苑生物
更新时间： 2025-03-31T12:35:07
使用范围： null
| 规格 | 价格 |
| 50 mL | 500.0 |
| 100 mL | 900.0 |
- 联系人 :
- 地址 : 江北新区智能制造产业园（智合园）科创大道9号A2栋
- 邮编 : 210000
- 所在区域 : 江苏
- 电话 : 177****6632 点击查看
- 传真 : 点击查看
- 邮箱 : jiangyuanbio@163.com
<p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">产品简介</span></strong></p> <p class="MsoNormal" style="font-size: medium; text-indent: 21pt;">聚乙二醇（<span lang="EN-US">PEG</span>）是环氧乙烷和水缩合而成的水溶性非离子型聚合物，相对分子质量为<span lang="EN-US">8000</span>的<span lang="EN-US">PEG</span>可用于浓缩病毒。<span lang="EN-US">PEG</span>浓缩法能成百倍的浓缩病毒，而且对病毒的结构和抗原性有保护作用。本产品（<span lang="EN-US">PEG-8000</span>慢病毒浓缩液）为<span lang="EN-US">5×</span>浓缩液，能简单快速地浓缩所有类型的慢病毒颗粒。</p> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">保存条件</span></strong></p> <p class="MsoNormal" style="font-size: medium;">常温配送。<span lang="EN-US">4 oC</span>保存，<span lang="EN-US">1</span>年有效。</p> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">注意事项</span></strong></p> <p class="MsoNormal" style="font-size: medium;"><span lang="EN-US">1</span>．本产品仅用于科学研究，不得存放于普通住宅内。</p> <p class="MsoNormal" style="font-size: medium;" align="left"><span lang="EN-US">2</span>．为了您的安全和健康，请穿实验服并戴一次性手套操作。</p> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">使用说明</span></strong></p> <p class="MsoNormal" style="font-size: medium;"><span lang="EN-US">1</span>．<span lang="EN-US">0.45 μm</span>滤头过滤慢病毒上清。</p> <p class="MsoNormal" style="font-size: medium; margin-left: 10.5pt; text-indent: -10.5pt;"><span lang="EN-US">2</span>．每<span lang="EN-US">30 mL</span>过滤后的病毒初始液中加入<span lang="EN-US">7.5 mL</span>浓缩液（<span lang="EN-US">4:1=</span>上清液：浓缩液）。</p> <p class="MsoNormal" style="font-size: medium; margin-left: 10.5pt; text-indent: -10.5pt;"><span lang="EN-US">3</span>．每<span lang="EN-US">20-30 min</span>混合一次，共混合<span lang="EN-US">3-5</span>次。</p> <p class="MsoNormal" style="font-size: medium; margin-left: 10.5pt; text-indent: -10.5pt;"><span lang="EN-US">4</span>．<span lang="EN-US">4°C</span>放置过夜。</p> <p class="MsoNormal" style="font-size: medium;"><span lang="EN-US">5</span>．于<span lang="EN-US">4°C</span>离心机<span lang="EN-US">4000 <em>g</em> </span>离心<span lang="EN-US">25 min</span>。</p> <p class="MsoNormal" style="font-size: medium;"><span lang="EN-US">6</span>．吸弃上清，静置<span lang="EN-US">1-2 min</span>，吸走残余液体。</p> <p class="MsoNormal" style="font-size: medium; margin-left: 10.5pt; text-indent: -10.5pt;"><span lang="EN-US">7</span>．加入适量<span lang="EN-US">PBS</span>重悬病毒颗粒。</p> <p class="MsoNormal" style="font-size: medium;"><span lang="EN-US">8</span>．测定浓缩后的慢病毒样品的滴度，<span lang="EN-US">50 μL/</span>管分装，于<span lang="EN-US">-80 °C</span>冰箱保存。</p> <p><strong><span style="color: #2e74b5;">相关产品</span></strong></p> <table width="643"> <tbody> <tr> <td width="189"> <p><span style="color: black;">产品编号</span></p> </td> <td width="284"> <p><span style="color: black;">产品名称</span></p> </td> <td width="170"> <p><span style="color: black;">产品规格</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY03011/JY03012</span></p> </td> <td width="284"> <p>慢病毒浓缩试剂盒</p> </td> <td width="170"> <p><span style="color: black;">50 mL/100 mL</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY03013/JY03014</span></p> </td> <td width="284"> <p>PEG-8000慢病毒浓缩液</p> </td> <td width="170"> <p><span style="color: black;">50 mL/100 mL</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY030611/JY03061/JY03062</span></p> </td> <td width="284"> <p>全套第二代慢病毒包装质粒（三质粒系统）</p> </td> <td width="170"> <p><span style="color: black;">1mL</span><span style="color: black;">甘油菌</span><span style="color: black;">/</span><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY030631/JY03063/JY03064</span></p> </td> <td width="284"> <p>全套第三代慢病毒包装质粒（四质粒系统）</p> </td> <td width="170"> <p><span style="color: black;">1mL</span><span style="color: black;">甘油菌</span><span style="color: black;">/</span><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY030271/JY03027/JY03028</span></p> </td> <td width="284"> <p>慢病毒包装质粒 pMD2.G</p> </td> <td width="170"> <p><span style="color: black;">1mL</span><span style="color: black;">甘油菌</span><span style="color: black;">/</span><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY030291/JY03029/JY03030</span></p> </td> <td width="284"> <p>慢病毒包装质粒 psPAX2</p> </td> <td width="170"> <p><span style="color: black;">1mL</span><span style="color: black;">甘油菌</span><span style="color: black;">/</span><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY030311/JY03031/JY03032</span></p> </td> <td width="284"> <p>慢病毒包装质粒 pCMV-VSV-G</p> </td> <td width="170"> <p><span style="color: black;">1mL</span><span style="color: black;">甘油菌</span><span style="color: black;">/</span><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY030331/JY03033/JY03034</span></p> </td> <td width="284"> <p>慢病毒包装质粒 pCMV-dR8.91</p> </td> <td width="170"> <p><span style="color: black;">1mL</span><span style="color: black;">甘油菌</span><span style="color: black;">/</span><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY030351/JY03035/JY03036</span></p> </td> <td width="284"> <p>慢病毒包装质粒 pMDLg/pRRE</p> </td> <td width="170"> <p><span style="color: black;">1mL</span><span style="color: black;">甘油菌</span><span style="color: black;">/</span><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY030371/JY03037/JY03038</span></p> </td> <td width="284"> <p>慢病毒包装质粒 pRSV-Rev</p> </td> <td width="170"> <p><span style="color: black;">1mL</span><span style="color: black;">甘油菌</span><span style="color: black;">/</span><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="189"> <p><span style="color: black;">JY03041</span></p> </td> <td width="284"> <p>293T，HEK-293T，人胚肾细胞</p> </td> <td width="170"> <p><span style="color: black;">> 1×10⁶ cells</span></p> </td> </tr> </tbody> </table>